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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A303-541A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USF2 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (T; 50 µg), and Jurkat (J; 50 µg) cells. Antibody: Affinity purified rabbit anti-USF2 antibody (Product # A303-541A; lot A303-541A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody A303-541A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies A303-540A and A303-542A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse USF2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- USF2 (Cat. No. A303-541A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USF2 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 (Cat. No. A303-540A Lot1, left image, A303-541A Lot1, middle image and Cat. No. A303-542A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human USF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USF2 antibody (Product # A303-541A; lot A303-541A-1) used for IP at 6 µg/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies (Product # A303-540A and Product # A303-542A), which recognize other epitopes. For blotting immunoprecipitated USF2 (Product # A303-541A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.
