MA5-24173

antibody from Invitrogen Antibodies

Targeting: PON3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24173

Provider

Invitrogen Antibodies

Product name

PON3 Monoclonal Antibody (454929)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON1 or rhPON2. Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human, Mouse, Rat

Host

Rat

Isotype

IgG

Antibody clone number

454929

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of human/mouse/Rat PON3 Monoclonal Antibody (Product # MA5-24173) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PON3 in human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. Samples were incubated in PON3 monoclonal antibody (Product # MA5-24173) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PON3 in human liver tissue, an enriched HDL /LDL sample obtained from ultracentrifugation of human plasma, mouse liver, and rat liver tissue. Samples were incubated in PON3 monoclonal antibody (Product # MA5-24173) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.