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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-35306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDAH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references DDAH1 regulates apoptosis and angiogenesis in human fetal pulmonary microvascular endothelial cells.
Trittmann JK, Almazroue H, Jin Y, Nelin LD
Physiological reports 2019 Jul;7(12):e14150
Physiological reports 2019 Jul;7(12):e14150
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDAH1 in HepG2 cells using a DDAH1 polyclonal antibody (Product # PA5-35306) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of DDAH1 in 293 cells (bottom) compared to a negative control (top) using a DDAH1 polyclonal antibody (Product # PA5-35306) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 siRNA-mediated knockdown of DDAH1 in hfPMVEC resulted in less NO production with no significant change in eNOS protein levels. (A) DDAH1 siRNA transfection of hfPMVECs resulted in effective knockdown of DDAH1 protein levels. hfPMVECs were transfected with either scramble siRNA or DDAH1 siRNA for 24 h, recovered for 24 h, and protein isolated. Representative western blot and bar graph of densitometry data for DDAH1 protein levels normalized to beta -actin ( n = 6 in each group). * P < 0.05, DDAH1 siRNA different from scramble. (B) DDAH1 knockdown with siRNA resulted in lower NO production than scramble transfected cells. hfPMVECs were transfected with either scramble siRNA or DDAH1 siRNA for 24 h, washed and allowed to recover for 24 h. Cell were incubated for an additional 24 h and cell media harvested for determination of nitrites using a chemiluminescence NO analyzer. Nitrite levels were normalized to protein concentration in each plate ( n = 3 in each group). * P < 0.05, DDAH1 siRNA different from scramble. (C) DDAH1 siRNA transfection of hfPMVECs resulted in no significant change of eNOS protein levels. The protein isolated under B above was used in western blotting for eNOS protein levels. Representative western blot and bar graph of densitometry data for eNOS protein level normalized to beta -actin ( n = 6 in each group).
