Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-96632 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TFEB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: Raji, NIH/3T3, mouse spleen, mouse kidney, rat brain, rat spleen; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: TPAINTPVHF QSPPPVPGEV LKVQSYLENP TSYHLQQSQH QKVREYLSET YGNKFAAHIS PAQGSPKPPP AASPGVRAGH VLSSSAGNSA PNSPMAMLHI GSNPERELDD VIDNIMRLDD VLGY
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.08 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TFEB in extracts of various cell lines using TFEB Polyclonal Antibody (Product # PA5-96632) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TFEB was performed in NIH/3T3 cells using TFEB Polyclonal Antibody (Product # PA5-96632).