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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A303-673A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TFEB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TFEB by western blot. Samples: Whole cell lysate (10 µg) from HeLa, Hep-G2, MCF-7, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody (Product # A303-673A lot 8) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse TFEB by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody (Product # A303-673A lot 8) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody A303-673A (lot A303-673A-5) used for IP at 6 µg per reaction. TFEB was also immunoprecipitated by a previous lot of this antibody (lot A303-673A-4) and rabbit anti-TFEB antibody A303-672A For blotting immunoprecipitated TFEB, A303-673A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse TFEB by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody A303-673A (lot A303-673A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse TFEB by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-TFEB (Cat. No. A303-673A Lot 5) used at 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TFEB by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Affinity purified rabbit anti-TFEB (Cat. No. A303-673A Lot 5) used at 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody (Product # A303-673A; Lot 8) used for IP at 6 µg per reaction. TFEB was also immunoprecipitated by a previous lot of this antibody (Product # A303-673A; Lot 7) and a second antibody against a different epitope of TFEB (Product # A303-672A). For blotting immunoprecipitated TFEB, Product # A303-673A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
