Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-77307 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotanalysis of rat heart membranes with TRPC3 polyclonal antibody (Product # PA5-77307) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TRPC3 in paraformaldehyde-fixed and permeabilized rat C6 brain glioma cells. A) Samples were probed with TRPC3 polyclonal antibody (Product # PA5-77307) at a dilution of 1:500, and incubated with goat-anti-rabbit-AlexaFluor-488 and Hoechst. B) Nuclei stained image. C) Merged images of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC3 in rat cerebellum. A) Samples were probed with TRPC3 polyclonal antibody (Product # PA5-77307) and incubated with parvalbumin (red) and DAPI. B) Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC3 in rat cerebellum. A) Samples were probed with TRPC3 polyclonal antibody (Product # PA5-77307) and incubated with parvalbumin (red) and DAPI. B) Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC3 in rat cerebellum. A) Samples were probed with TRPC3 polyclonal antibody (Product # PA5-77307) and incubated with parvalbumin (red) and DAPI. B) Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPC3 in rat cerebellum. A) Samples were probed with TRPC3 polyclonal antibody (Product # PA5-77307) and incubated with parvalbumin (red) and DAPI. B) Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining.