H00008659-M01

antibody from Abnova Corporation

Targeting: ALDH4A1 ALDH4, P5CDh

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008659-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008659-M01, RRID:AB_425775

Product name

ALDH4A1 monoclonal antibody (M01), clone 1A12-A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ALDH4A1.

Antigen sequence

MLLPAPALRRALLSRPWTGAGLRWKHTSSLKVANE
PVLAFTQGSPERDALQKALKDLKGRMEAIPCVVGD
EEVWTSDVQYQVSPFNHGHKVAKFCYADKSLLNKA
IEAALAARKEWDLKPIADRAQIFLKAADMLSGPRR
AEILAKTMVGQGKTVIQAEIDAAAELIDFFRFNAK
YAVELEGQQPISVPPSTNSTVYRGLEGFVAAISPF
NFTAIGGNLAGAPALMGNVVLWKPSDTAMLASYAV
YRILREAGLPPNIIQFVPADGPLFGDTVTSSEHLC
GINFTGSVPTFKHLWKQVAQNLDRFHTFPRLAGEC
GGKNFHFVHRSADVESVVSGTLRSAFEYGGQKCSA
CSRLYVPHSLWPQIKGRLLEEHSRIKVGDPAEDFG
TFFSAVIDAKSFARIKKWLEHARSSPSLTILAGGK
CDDSVGYFVEPCIVESKDPQEPIMKEEIFGPVLSV
YVYPDDKYKETLQLIDSTTSYGLTGAVFSQDKDVV
QEATKVLRNAAGNFYINDKSTGSIVGQQPFGGARA
SGTNDKPGGPHYILRWTSPQVIKETHKPLGDWSYA
YMQ


Isotype

IgG

Antibody clone number

1A12-A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ALDH4A1 expression in transfected 293T cell line by ALDH4A1 monoclonal antibody (M01), clone 1A12-A5.Lane 1: ALDH4A1 transfected lysate(61.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ALDH4A1 monoclonal antibody (M01), clone 1A12-A5. Western Blot analysis of ALDH4A1 expression in A-431(Cat # L015V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ALDH4A1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ALDH4A1 on formalin-fixed paraffin-embedded human liver tissue.[antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol