MA5-29027

antibody from Invitrogen Antibodies

Targeting: ALDH4A1 ALDH4, P5CDh

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29027

Provider

Invitrogen Antibodies

Product name

ALDH4A1 Recombinant Rabbit Monoclonal Antibody (2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

2

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ALDH4A1 in Lane A: HepG2 Whole Cell Lysate (30 µg), Lane B: K562 Whole Cell Lysate (30 µg), Lane C: A549 Whole Cell Lysate (30 µg). Samples were probed using a ALDH4A1 Monoclonal Antibody (Product # MA5-29027) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:62 kDa. Observed band size:62 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ALDH4A1 Recombinant Rabbit Monoclonal Antibody (2) (Product # MA5-29027) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ALDH4A1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg K562 Whole Cell Lysate 2 µL with ALDH4A1 Recombinant Rabbit Monoclonal Antibody (2) (Product # MA5-29027) and 15 µL of 50 % Protein G agarose. Primary antibody: ALDH4A1 Recombinant Rabbit Monoclonal Antibody (2), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa.