H00005166-M04

antibody from Abnova Corporation

Targeting: PDK4

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005166-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005166-M04, RRID:AB_894232

Product name

PDK4 monoclonal antibody (M04), clone 1A10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PDK4.

Antigen sequence

MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPL
SMKQLLDFGSENACERTSFAFLRQELPVRLANILK
EIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEK
SPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIE
YKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQ
HILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFEC
SRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVP
SHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIV
VLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAP
TPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNL
YSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKH
YQMSSEADDWCIPSREPKNLAKEVAM


Isotype

IgG

Antibody clone number

1A10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Alteration of energy metabolism in the pathogenesis of bile duct lesions in primary biliary cirrhosis.

Harada K, Kakuda Y, Sato Y, Ikeda H, Shimoda S, Yamamoto Y, Inoue H, Ohta H, Kasashima S, Kawashima A, Nakanuma Y
Journal of clinical pathology 2014 May;67(5):396-402

---

The increase in cardiac pyruvate dehydrogenase kinase-4 after short-term dexamethasone is controlled by an Akt-p38-forkhead box other factor-1 signaling axis.

Puthanveetil P, Wang Y, Wang F, Kim MS, Abrahani A, Rodrigues B
Endocrinology 2010 May;151(5):2306-18

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PDK4 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol