PA5-56830

antibody from Invitrogen Antibodies

Targeting: PGC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56830

Provider

Invitrogen Antibodies

Product name

PGC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VPLKKFKSIR ETMKEKGLLG EFLRTHKYDP AWKYRFGDLS VTYEPMAYMD AAYFGEISIG T

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGC in control (vector only transfected HEK293T lysate) and PGC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a PGC Polyclonal Antibody (Product # PA5-56830).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human stomach and skeletal muscle tissues using PGC Polyclonal Antibody (Product # PA5-56830). Corresponding PGC RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human cerebral cortex, colon, skeletal muscle and stomach using PGC Polyclonal Antibody (Product # PA5-56830) (A) shows similar protein distribution across tissues to an independent PGC Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human stomach using PGC Polyclonal Antibody (Product # PA5-56830) shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human skeletal muscle using PGC Polyclonal Antibody (Product # PA5-56830) shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human cerebral cortex using PGC Polyclonal Antibody (Product # PA5-56830) shows no positivity in neurons as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PGC in human colon using PGC Polyclonal Antibody (Product # PA5-56830) shows no positivity in glandular cells as expected.