AFR-015-200UL

antibody from Invitrogen Antibodies

Targeting: FFAR2 FFA2R, GPR43

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AFR-015-200UL

Provider

Invitrogen Antibodies

Product name

GPR43/FFAR2 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human HT-29 colon adenocarcinoma cell line (lanes 1 and 5), human HL-60 promyelocytic leukemia cell line (lanes 2 and 6), human THP-1 monocytic leukemia cell line (lane 3 and 7) and human LoVo colorectal adenocarcinoma cell line (lanes 4 and 8)lysates: - 1-4. Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015), (1:200). 5-8. Anti-Human GPR43/FFAR2 (extracellular) Antibody , preincubated with Human GPR43/FFAR2 (extracellular) Blocking Peptide (#BLP-FR015).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of FFAR2 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015), (2.5 µg) + goat- Anti-rabbit-FITC.