TA335689

antibody from OriGene

Targeting: CCT4 Cctd

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA335689

Provider

OriGene

Product name

Rabbit Polyclonal Anti-CCT4 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-CCT4 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

CCT4

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB Suggested Anti-CCT4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500 Positive Control: HepG2 cell lysate; CCT4 is supported by BioGPS gene expression data to be expressed in HepG2

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

CCT4 antibody - C-terminal region validated by WB using 293T cells lysate; CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: Lane1: Negative control (anti-FLAG antibody); Lane2: Immunoprecipitation with CCT4 (C-term); Lane3: 10 ug lysate; Primary Antibody Dilution: ; 1:1000; Secondary Antibody: ; Goat anti-rabbit; Secondary Antibody Dilution: ; 1:10000; Gene Name: ; CCT4; Submitted by: ; Amy Gray, Department of Chemistry and Biochemistry Brigham Young University; CCT4 is supported by BioGPS gene expression data to be expressed in HEK293T

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample Type : Rat Hippocampal Neurons - 14DIV Primary Antibody Dilution : 1:200 Secondary Antibody : Anti-rabbit-Cy3 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions : White: CCT4 Gene Name : CCT4 Submitted by : Dan Fowler - University of Oregon, Institute of Neuroscience

Validation comment

IHC