PA5-93048

antibody from Invitrogen Antibodies

Targeting: CCT4 Cctd

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93048

Provider

Invitrogen Antibodies

Product name

TCP-1 delta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PTIISESFQK ALEKGIEILT DMSRPVELSD RETLLNSATT SLNSKVVSQY SSLLSPMSVN AVMKVIDPAT ATSVDLRDIK IVKKLGGTID DCELVEGLVL TQKVSNSGIT RVEKAKIGLI QFCLSAPKTD MDNQIVVSDY AQMDRVLREE RAYILNLVKQ IKKTGCNVLL IQKSILRDAL SDLALHFLNK MKIMVIKDIE REDIEFICKT IGTKPVAHID QFTADMLGSA ELAEEVNLNG SGKLLKITGC ASPGKTVTIV V; Positive Samples: MCF7, THP-1, Mouse spleen; Cellular Location: Cytoplasm, Melanosome, centrosome, cilium basal body, cytoskeleton, microtubule organizing center

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.35 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CCT4 Polyclonal antibody (Product # PA5-93048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TCP-1 delta in extracts of various cell lines using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TCP-1 delta in extracts of various cell lines using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TCP-1 delta was performed in HeLa cells using TCP-1 delta Polyclonal Antibody (Product # PA5-93048).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCP-1 delta in paraffin-embedded rat stomach using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCP-1 delta in paraffin-embedded mouse testis using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCP-1 delta in paraffin-embedded mouse spinal cord using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TCP-1 delta in paraffin-embedded mouse brain using TCP-1 delta Polyclonal Antibody (Product # PA5-93048) at a dilution of 1:200.