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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-21991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, rat liver. Predicted reactivity: Mouse (96%), Xenopus laevis (88%), Chicken (94%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer.
Miyo M, Konno M, Nishida N, Sueda T, Noguchi K, Matsui H, Colvin H, Kawamoto K, Koseki J, Haraguchi N, Nishimura J, Hata T, Gotoh N, Matsuda F, Satoh T, Mizushima T, Shimizu H, Doki Y, Mori M, Ishii H
Scientific reports 2016 Dec 7;6:38415
Scientific reports 2016 Dec 7;6:38415
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A13 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SLC25A13 polyclonal antibody (Product # PA5-21991) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC25A13 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLC25A13 Polyclonal Antibody (Product # PA5-21991) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLC25A13 Polyclonal Antibody (Product # PA5-21991). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 7.5% SDS PAGE. SLC25A13 Polyclonal Antibody (Product # PA5-21991) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLC25A13 Polyclonal Antibody (Product # PA5-21991). Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 Polyclonal Antibody (Product # PA5-21991) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SLC25A13 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A13 Polyclonal Antibody (Product # PA5-21991) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SLC25A13 was performed in paraffin-embedded mouse kidney tissue using SLC25A13 Polyclonal Antibody (Product # PA5-21991) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SLC25A13 was performed in paraffin-embedded mouse kidney tissue using SLC25A13 Polyclonal Antibody (Product # PA5-21991) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SLC25A13 (Product # PA5-21991) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
