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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-80698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HTR4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has specificity for Human HTR4/5-HT4.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HTR4 in Lane A: SW480 Whole Cell Lysate (30 µg), Lane B: HeLa Whole Cell Lysate (30 µg). Samples were probed using a HTR4 Polyclonal Antibody (Product # PA5-80698) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), HRP Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:43 kDa. Observed band size:44 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HTR4 Polyclonal Antibody (Product # PA5-80698) at 1:500 dilution. Lane A: SW480 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HTR4 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with HTR4 Polyclonal Antibody (Product # PA5-80698) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HTR4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.
