Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF2800 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse pIgR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O70570
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells.
Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation.
Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.
Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA.
Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease.
Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation.
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
Bornstein C, Nevo S, Giladi A, Kadouri N, Pouzolles M, Gerbe F, David E, Machado A, Chuprin A, Tóth B, Goldberg O, Itzkovitz S, Taylor N, Jay P, Zimmermann VS, Abramson J, Amit I
Nature 2018 Jul;559(7715):622-626
Nature 2018 Jul;559(7715):622-626
Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation.
Basic M, Buettner M, Keubler LM, Smoczek A, Bruesch I, Buchheister S, Bleich A
Scientific reports 2018 Jan 15;8(1):719
Scientific reports 2018 Jan 15;8(1):719
Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.
Azzam KM, Madenspacher JH, Cain DW, Lai L, Gowdy KM, Rai P, Janardhan K, Clayton N, Cunningham W, Jensen H, Patel PS, Kearney JF, Taylor GA, Fessler MB
JCI insight 2017 Aug 17;2(16)
JCI insight 2017 Aug 17;2(16)
Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA.
Nakanishi K, Morikane S, Ichikawa S, Kurohane K, Niwa Y, Akimoto Y, Matsubara S, Kawakami H, Kobayashi H, Imai Y
Scientific reports 2017 Apr 3;7:45843
Scientific reports 2017 Apr 3;7:45843
Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
Moon C, Baldridge MT, Wallace MA, D CA, Burnham, Virgin HW, Stappenbeck TS
Nature 2015 May 7;521(7550):90-93
Nature 2015 May 7;521(7550):90-93
Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease.
Olsan EE, Matsushita T, Rezaei M, Weimbs T
The Journal of biological chemistry 2015 Jun 19;290(25):15679-86
The Journal of biological chemistry 2015 Jun 19;290(25):15679-86
Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation.
Hegab AE, Ha VL, Bisht B, Darmawan DO, Ooi AT, Zhang KX, Paul MK, Kim YS, Gilbert JL, Attiga YS, Alva-Ornelas JA, Nickerson DW, Gomperts BN
Stem cells and development 2014 Mar 15;23(6):664-75
Stem cells and development 2014 Mar 15;23(6):664-75
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
Moon C, VanDussen KL, Miyoshi H, Stappenbeck TS
Mucosal immunology 2014 Jul;7(4):818-28
Mucosal immunology 2014 Jul;7(4):818-28
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomarker insights 2012;7:87-104
Biomarker insights 2012;7:87-104
Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
Frantz AL, Rogier EW, Weber CR, Shen L, Cohen DA, Fenton LA, Bruno ME, Kaetzel CS
Mucosal immunology 2012 Sep;5(5):501-12
Mucosal immunology 2012 Sep;5(5):501-12
Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN
Vaccine 2010 Mar 8;28(11):2311-7
Vaccine 2010 Mar 8;28(11):2311-7
The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
Libanova R, Ebensen T, Schulze K, Bruhn D, Nörder M, Yevsa T, Morr M, Guzmán CA
Vaccine 2010 Mar 2;28(10):2249-2258
Vaccine 2010 Mar 2;28(10):2249-2258
Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA
Proteomics 2010 Feb;10(3):520-31
Proteomics 2010 Feb;10(3):520-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse pIgR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- pIgR in Mouse Liver. pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.