MA5-37944

antibody from Invitrogen Antibodies

Targeting: CTRL

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-37944

Provider

Invitrogen Antibodies

Product name

CTRL Recombinant Rabbit Monoclonal Antibody (10Z4K3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: Mouse pancreas, Rat pancreas Immunogen sequence: GVGNVTPAHL QQVALPLVTV NQCRQYWGSS ITDSMICAGG AGASSCQGDS GGPLVCQKGN TWVLIGIVSW GTKNCNVRAP AVYTRVSKFS TWINQVIAYN

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

10Z4K3

Vial size

100 μL

Concentration

0.3 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CTRL in extracts of various cell lines. Samples were incubated with CTRL Monoclonal antibody (Product # MA5-37944) using a dilution of 1:5,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CTRL in rat pancreas cells. Samples were incubated with CTRL Monoclonal antibody (Product # MA5-37944) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CTRL in mouse pancreas cells. Samples were incubated with CTRL Monoclonal antibody (Product # MA5-37944) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CTRL in paraffin-embedded rat pancreas. Samples were incubated with CTRL Monoclonal antibody (Product # MA5-37944) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CTRL in paraffin-embedded mouse pancreas. Samples were incubated with CTRL Monoclonal antibody (Product # MA5-37944) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.