GTX131112

antibody from GeneTex

Targeting: PBK CT84, FLJ14385, Nori-3, SPK, TOPK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX131112

Provider

GeneTex

Product name

PBK antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (GTX131112) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (GTX131112) diluted at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PBK antibody detects PBK protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PBK protein stained by PBK antibody (GTX131112) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 £gg of PBK antibody (GTX131112).Western blot analysis was performed using PBK antibody (GTX131112).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.