Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-38403 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-beta-2 Adrenergic Receptor (Ser355, Ser356) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Epistatic interaction of PDE4DIP and DES mutations in familial atrial fibrillation with slow conduction.
Light Entrains Diurnal Changes in Insulin Sensitivity of Skeletal Muscle via Ventromedial Hypothalamic Neurons.
Abou Ziki MD, Bhat N, Neogi A, Driscoll TP, Ugwu N, Liu Y, Smith E, Abboud JM, Chouairi S, Schwartz MA, Akar JG, Mani A
Human mutation 2021 Oct;42(10):1279-1293
Human mutation 2021 Oct;42(10):1279-1293
Light Entrains Diurnal Changes in Insulin Sensitivity of Skeletal Muscle via Ventromedial Hypothalamic Neurons.
Aras E, Ramadori G, Kinouchi K, Liu Y, Ioris RM, Brenachot X, Ljubicic S, Veyrat-Durebex C, Mannucci S, GaliƩ M, Baldi P, Sassone-Corsi P, Coppari R
Cell reports 2019 May 21;27(8):2385-2398.e3
Cell reports 2019 May 21;27(8):2385-2398.e3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AR-beta-2 pSer355/pSer356 in extracts from HUVEC cells and COS7 cells treated with serum (20%, 15min) using a Phospho-AR-beta-2 pSer355/pSer356 polyclonal antibody (Product # PA5-38403).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-AR-beta-2 pSer355/pSer356 in paraffin-embedded human brain tissue using a Phospho-AR-beta-2 pSer355/pSer356 polyclonal antibody (Product # PA5-38403).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Figure Western blot analysis of beta2 adrenergic receptor (beta2AR) phosphorylation. (a) Phosphorylation at the PKA site (p-Ser 346) in the beta2AR before (-iso) and 8 min after 1 uM of isoproterenol stimulation (+iso) in C2C12 cells transfected with mCherry-empty plasmid, or wild type mCherry-PDE4DIP (WT) plasmid, or mutant mCherry-PDE4DIP (mut) plasmid, also shown is the corresponding total beta2AR and a PonceauS protein stain as a loading control. (b) Phosphorylation at the GRK sites (p-Ser 355,356) in the beta2AR before (-iso) and 8 min after 1 uM of isoproterenol stimulation (+iso), and the corresponding total beta2AR, also shown is beta-actin as an additional protein loading control, of note all the Western blots (a and b) were loaded with the identical amount of lysate. (c) The ratio of phosphorylated beta2AR at the PKA site (Ser346) to the total beta2AR. There is increased PKA phosphorylation at baseline in the wild type PDE4DIP compared with the control and the mutant however, after isoproterenol stimulation, there is a much higher levels of PKA mediated phosphorylation of the beta2AR in the mutant compared with wild type PDE4DIP transfected cells, there is also an increase in PKA phosphorylation of the beta2AR in the control cells. (d) The ratio of phosphorylated beta2AR at the GRK sites (Ser355,356) to the total beta2AR. There is increased PKA phosphorylation at baseline in the wild type PDE4DIP compared with the control and the mutant, however after isoprotereno