PA5-18423

antibody from Invitrogen Antibodies

Targeting: ADRB2 ADRB2R, ADRBR, B2AR, BAR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18423

Provider

Invitrogen Antibodies

Product name

beta-2 Adrenergic Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine based on sequence homology.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human liver lysate using Product # PA5-18423 at a concentration of 0.2 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-beta-2 Adrenergic Receptor Polyclonal Antibody, (Product # PA5-18423) and a 50 kDa band corresponding to ADRB2 was observed in the cell lines tested. Membrane enriched cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), BeWo (Lane 3), OVCAR-3 (Lane 4), and SK-OV-3 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.25 µg/mL concentration) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).