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- Product number
- ADI-905-742-100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-905-742-100, RRID:AB_2038871
- Product name
- Adrenergic receptor _2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Keep on ice at all times.
Submitted references Conformation state-sensitive antibodies to G-protein-coupled receptors.
Gupta A, Décaillot FM, Gomes I, Tkalych O, Heimann AS, Ferro ES, Devi LA
The Journal of biological chemistry 2007 Feb 23;282(8):5116-24
The Journal of biological chemistry 2007 Feb 23;282(8):5116-24
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: MW marker (1) and 20µg rat heart membrane extract (2) probed with Adrenergic Receptor (_2) pAb at 2.5µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis: Fluorescent confocal photomicrograph from rat brainstem. Neurons are labeled with Adrenergic Receptor (_2) pAb and Cy3 conjugated rabbit IgG antibody (H+L).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Membrane ELISA: Lewis rat brain membranes (5µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with Adrenergic Receptor (_2) pAb (1:1000 of a stock solution of 1µg/µL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Cellular ELISA: HEK293 cells expressing Adrenergic Receptor _2 were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with Adrenergic Receptor (_2) pAb (1:1000 of a stock solution of 1µg/µL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
