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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-22965 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LCAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- Suggested positive control: antigen standard for LCAT (transient overexpression lysate), Lipoprotein fraction of whole mouse plasma.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LCAT in 0.2 µL four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT. Samples were incubated in LCAT polyclonal antibody (Product # PA5-22965). purified mouse LCAT were fractionated on SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-LCAT Polyclonal Antibody (Product # PA5-22965) and a band around 65 kDa corresponding to LCAT was observed in Hep G2 but not in SH-SY5Y. However, SH-SY5Y cells treated with CBM showed the band for LCAT. Whole cell extracts (40 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with CBM (Lane 2), SH-SY5Y (Lane 3) or SH-SY5Y treated with CBM (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
