Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-52599 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGFG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PQSTATANFA NFAHFNSHAA QNSANADFAN FDAFGQSSGS SNFGGFPTAS HSPFQPQTTG GSAASVNANF AHFDNFPKSS SADFGTFNTS QSHQTASAVS KVSTNKAGLQ TADKYAALAN LDNIFSAG Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 94%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGFG1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an AGFG1 Polyclonal Antibody (Product # PA5-52599).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGFG1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an AGFG1 Polyclonal Antibody (Product # PA5-52599).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of AGFG1 was achieved by transfecting A549 cells with AGFG1 specific siRNAs (Silencer® select Product # s6896, s6897). Western blot analysis (Fig. a) was performed using whole cell extracts from the AGFG1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed AGFG1 Polyclonal Antibody (Product # PA5-52599, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to AGFG1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-AGFG1 Polyclonal Antibody (Product # PA5-52599) and a 60kDa band corresponding to AGFG1 was observed across samples tested. Uncharacterized bands (*), corresponding to ~47kDa and ~38kDa were observed in all cell lines along with ~115kDa in HeLa, IMR32, K562 and Mouse Testis only. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2), K-562 (Lane 3), A549 (Lane 4), PC-12 (Lane 5) and Mouse Testis (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of AGFG1 in human cell line A-431 shows positivity in vesicles. Samples were probed using an AGFG1 Polyclonal Antibody (Product # PA5-52599).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGFG1 in human placenta shows strong membranous positivity in trophoblastic cells. Samples were probed using an AGFG1 Polyclonal Antibody (Product # PA5-52599).