15904-1-AP

antibody from Proteintech Group

Targeting: MDH1

Provider product page for 15904-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 15904-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15904-1-AP, RRID:AB_2143279
Product name: MDH1 antibody
Antibody type: Polyclonal
Description: KD/KO validated MDH1 antibody (Cat. #15904-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MDH1 protein structure - 15904-1-AP shown in red.

Submitted references The acetylation of MDH1 and IDH1 is associated with energy metabolism in acute liver failure.

Shi C, Zhang Y, Chen Q, Wang Y, Zhang D, Guo J, Zhang Q, Zhang W, Gong Z
iScience 2024 May 17;27(5):109678

IDH1/MDH1 deacetylation promotes acute liver failure by regulating NETosis.

Wang Y, Shi C, Guo J, Zhang D, Zhang Y, Zhang L, Gong Z
Cellular & molecular biology letters 2024 Jan 3;29(1):8

Exploration of spatial heterogeneity of tumor microenvironment in nasopharyngeal carcinoma via transcriptional digital spatial profiling.

Wang L, Wang D, Zeng X, Zhang Q, Wu H, Liu J, Wang Y, Liu G, Pan Y
International journal of biological sciences 2023;19(7):2256-2269

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway.

Mai L, He G, Chen J, Zhu J, Chen S, Yang H, Zhang M, Hou X, Ke M, Li X
Diabetes, metabolic syndrome and obesity : targets and therapy 2023;16:1731-1743

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Li F, Fu X, Luo X, Lin Q, Liang H, Niu Y, Liu L, Li N
Frontiers in microbiology 2023;14:1165491

Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells.

Hart ML, Quon E, Vigil ABG, Engstrom IA, Newsom OJ, Davidsen K, Hoellerbauer P, Carlisle SM, Sullivan LB
eLife 2023 Mar 8;12

HPV18 E6 inhibits α-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway.

Tang D, Zheng Y, Wang G, Sheng C, Liu Z, Wang B, Zong Q, Zhang Y, Hou X, Yao M, Zhou Z
FEBS open bio 2023 Aug;13(8):1522-1535

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation.

Li MX, Wu XT, Jing WQ, Hou WK, Hu S, Yan W
Cell death & disease 2023 Aug 2;14(8):492

Tat-malate dehydrogenase fusion protein protects neurons from oxidative and ischemic damage by reduction of reactive oxygen species and modulation of glutathione redox system.

Kwon HJ, Hahn KR, Kang MS, Choi JH, Moon SM, Yoon YS, Hwang IK, Kim DW
Scientific reports 2023 Apr 6;13(1):5653

Malonylation of Acetyl-CoA carboxylase 1 promotes hepatic steatosis and is attenuated by ketogenic diet in NAFLD.

Cao H, Cai Q, Guo W, Su Q, Qin H, Wang T, Xian Y, Zeng L, Cai M, Guan H, Chen S, Liang H, Xu F
Cell reports 2023 Apr 25;42(4):112319

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Fan TW, Daneshmandi S, Cassel TA, Uddin MB, Sledziona J, Thompson PT, Lin P, Higashi RM, Lane AN
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690

Comprehensive Analysis of Protein Acetylation and Glucose Metabolism in Mouse Brains Infected with Rabies Virus.

Pei J, Yuan Y, Tian D, Huang F, Zhang C, Wang C, Zhou M, Chen H, Fu Z, Zhao L
Journal of virology 2022 Feb 23;96(4):e0194221

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Zhang JY, Zhou B, Sun RY, Ai YL, Cheng K, Li FN, Wang BR, Liu FJ, Jiang ZH, Wang WJ, Zhou D, Chen HZ, Wu Q
Cell research 2021 Sep;31(9):980-997

iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats.

Wang D, Yu H, Li Y, Xu Z, Shi S, Dou D, Sun L, Zheng Z, Shi X, Deng X, Zhong X
Experimental and therapeutic medicine 2021 Sep;22(3):1014

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)

MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.

Gu H, Chen C, Hao X, Su N, Huang D, Zou Y, Lin SH, Chen X, Zheng D, Liu L, Yu Z, Xie L, Zhang Y, He X, Lai X, Zhang X, Chen GQ, Zhao Y, Yang Y, Loscalzo J, Zheng J
Blood 2020 Jul 30;136(5):553-571

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Sinha T, Naash MI, Al-Ubaidi MR
iScience 2020 Apr 24;23(4):101004

Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility.

Guo Y, Jiang W, Yu W, Niu X, Liu F, Zhou T, Zhang H, Li Y, Zhu H, Zhou Z, Sha J, Guo X, Chen D
Journal of proteomics 2019 Sep 30;208:103478

iTRAQ-Based Proteomic Analysis Reveals Recovery of Impaired Mitochondrial Function in Ischemic Myocardium by Shenmai Formula.

Wang Y, Zhao Y, Jiang W, Zhao X, Fan G, Zhang H, Shen P, He J, Fan X
Journal of proteome research 2018 Feb 2;17(2):794-803

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Wu L, Cao KX, Ni ZH, Li WD, Chen ZP, Cheng HB, Liu X
Journal of ethnopharmacology 2018 Aug 10;222:71-78

Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa.

Dai J, Xu W, Zhao X, Zhang M, Zhang D, Nie D, Bao M, Wang Z, Wang L, Qiao Z
Reproduction (Cambridge, England) 2016 Mar;151(3):227-37

An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.

Birsoy K, Wang T, Chen WW, Freinkman E, Abu-Remaileh M, Sabatini DM
Cell 2015 Jul 30;162(3):540-51

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Ma X, Li C, Sun L, Huang D, Li T, He X, Wu G, Yang Z, Zhong X, Song L, Gao P, Zhang H
Nature communications 2014 Oct 10;5:5212

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP(MDH1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line