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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C376102 - Provider product page
- Provider
- LSBio
- Product name
- MDH1 Antibody (aa1-334) LS-C376102
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: Malate dehydrogenase, cytoplasmic antibody at 4 ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: mouse kidney tissue. secondary Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All lanes: MDH1 antibody at 4µg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 28, 39 kDa Observed band size: 37 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating MDH1 in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of MDH1 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: MDH1 Antibody (4µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating MDH1 in HepG2 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of MDH1 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: MDH1 Antibody (4µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin tissue using antibody at 1:100 dilution.
