15904-1-AP

antibody from Invitrogen Antibodies

Targeting: MDH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15904-1-AP

Provider

Invitrogen Antibodies

Product name

MDH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSEPIRVLV TGAAGQIAYS LLYSIGNGSV FGKDQPIILV LLDITPMMGV LDGVLMELQD CALPLLKDVI ATDKEDVAFK DLDVAILVGS MPRREGMERK DLLKANVKIF KSQGAALDKY AKKSVKVIVV GNPANTNCLT ASKSAPSIPK ENFSCLTRLD HNRAKAQIAL KLGVTANDVK NVIIWGNHSS TQYPDVNHAK VKLQGKEVGV YEALKDDSWL KGEFVTTVQQ RGAAVIKARK LSSAMSAAKA ICDHVRDIWF GTPEGEFVSM GVISDGNSYG VPDDLLYSFP VVIKNKTWKF VEGLPINDFS REKMDLTAKE LTEEKESAFE FLSSA (1-334 aa encoded by BC001484)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of MDH1 antibody (15904-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human spleen tissue were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP ( MDH1 Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP ( MDH1 Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug MDH1 antibody (15904-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:1000) with mouse liver tissue lysate 6400ug.