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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005174-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005174-M01, RRID:AB_425585
- Product name
- PDZK1 monoclonal antibody (M01), clone 1C3-2B11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PDZK1.
- Antigen sequence
MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLV
RVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQ
VVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKEL
GQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVK
EGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVL
ADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFL
LVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVE
MKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEA
GLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQT
SLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVK
EAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGE
NGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDE
DVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVC
GKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVV
ESNHDSHMAKERAHSTASHSSSNSEDTEM- Isotype
- IgG
- Antibody clone number
- 1C3-2B11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Estrogen-regulated gene expression predicts response to endocrine therapy in patients with ovarian cancer.
Walker G, MacLeod K, Williams AR, Cameron DA, Smyth JF, Langdon SP
Gynecologic oncology 2007 Sep;106(3):461-8
Gynecologic oncology 2007 Sep;106(3):461-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PDZK1 monoclonal antibody (M01), clone 1C3-2B11. Western Blot analysis of PDZK1 expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PDZK1 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to PDZK1 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
