PA5-52261

antibody from Invitrogen Antibodies

Targeting: PDZK1 NHERF3, PDZD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52261

Provider

Invitrogen Antibodies

Product name

PDZK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MTSTFNPREC KLSKQEGQNY GFFLRIEKDT EGHLVRVVEK CSPAEKAGLQ DGDRVLRING VFVDKEEHMQ VVDLVRKSGN SVTLLVLDGD SYEKAV Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 92%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDZK1 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using a PDZK1 Polyclonal Antibody (Product # PA5-52261). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDZK1 in mouse liver tissue and rat liver tissue using a PDZK1 Polyclonal Antibody (Product # PA5-52261).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PDZK1 in human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a PDZK1 Polyclonal Antibody (Product # PA5-52261).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PDZK1 in human cell line RPTEC TERT1 using PDZK1 Polyclonal Antibody (Product # PA5-52261) shows localization to nucleus, cytosol & vesicles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PDZK1 in human kidney and tonsil tissues using PDZK1 Polyclonal Antibody (Product # PA5-52261). Corresponding PDZK1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PDZK1 in human kidney using PDZK1 Polyclonal Antibody (Product # PA5-52261) shows moderate to strong positivity in apical membrane in cells in proximal tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PDZK1 in human small intestine using PDZK1 Polyclonal Antibody (Product # PA5-52261) shows moderate to strong positivity in luminal membrane in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PDZK1 in human pancreas using PDZK1 Polyclonal Antibody (Product # PA5-52261) shows weak to moderate positivity in plasma membrane in islets of Langerhans.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PDZK1 in human tonsil using PDZK1 Polyclonal Antibody (Product # PA5-52261) shows no positivity as expected.