PAB28641

antibody from Abnova Corporation

Targeting: RHOJ ARHJ, FLJ14445, RASL7B, TCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28641

Provider

Abnova Corporation

Product name

RHOJ polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RHOJ.

Antigen sequence

CFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGT
QIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIG
AQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKR
CSEG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with RHOJ polyclonal antibody (Cat # PAB28641).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with RHOJ polyclonal antibody (Cat # PAB28641) at 1-4 ug/ml shows positivity in nucleus & nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with RHOJ polyclonal antibody (Cat # PAB28641) shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts and Leydig cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)