H00057381-M01

antibody from Abnova Corporation

Targeting: RHOJ ARHJ, FLJ14445, RASL7B, TCL

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00057381-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00057381-M01, RRID:AB_426036

Product name

RHOJ monoclonal antibody (M01), clone 1E4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RHOJ.

Antigen sequence

MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKT
CLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHL
LGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNP
ASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDD
PKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLEC
SALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSC
CSII


Isotype

IgG

Antibody clone number

1E4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

RhoJ regulates melanoma chemoresistance by suppressing pathways that sense DNA damage.

Ho H, Aruri J, Kapadia R, Mehr H, White MA, Ganesan AK
Cancer research 2012 Nov 1;72(21):5516-28

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Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice.

Fukushima Y, Okada M, Kataoka H, Hirashima M, Yoshida Y, Mann F, Gomi F, Nishida K, Nishikawa S, Uemura A
The Journal of clinical investigation 2011 May;121(5):1974-85

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RHOJ is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RHOJ on HeLa cell. [antibody concentration 60 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol