NBP1-86787

antibody from Novus Biologicals

Targeting: MRPS22 C3orf5, GIBT, GK002, MRP-S22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86787

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86787, RRID:AB_11026187

Product name

Rabbit Polyclonal MRPS22 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat MRPS22 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Mitochondria "fuel" breast cancer metabolism: fifteen markers of mitochondrial biogenesis label epithelial cancer cells, but are excluded from adjacent stromal cells.

Sotgia F, Whitaker-Menezes D, Martinez-Outschoorn UE, Salem AF, Tsirigos A, Lamb R, Sneddon S, Hulit J, Howell A, Lisanti MP
Cell cycle (Georgetown, Tex.) 2012 Dec 1;11(23):4390-401

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MRPS22 Antibody [NBP1-86787] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MRPS22 Antibody [NBP1-86787] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MRPS22 Antibody [NBP1-86787] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MRPS22 Antibody [NBP1-86787] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human kidney.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human cerebral cortex, colon, kidney and testis using Anti-MRPS22 antibody NBP1-86787 (A) shows similar protein distribution across tissues to independent antibody NBP1-86788 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MRPS22 Antibody [NBP1-86787] - Staining of human colon.