10960-1-AP

antibody from Proteintech Group

Targeting: CFL1 CFL

Provider product page for 10960-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [39]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

Submit

Product number: 10960-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10960-1-AP, RRID:AB_2291830
Product name: Cofilin antibody
Antibody type: Polyclonal
Description: KD/KO validated Cofilin antibody (Cat. #10960-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CFL1 protein structure - 10960-1-AP shown in red.

Submitted references Mechanism of Gzma-mediated GEF-H1 activation in intestinal epithelial cells leading to intestinal barrier dysfunction in sepsis.

Lin Z, Jiang H, Ni C, Deng L, Yang H, Wang R, Liu P, Li X, Yu Y, Li W, Liao B, Liu J, Li W, Yang J, Chao Y, Liu H, Wu X, Ren J, Zhao Y
Clinical and translational medicine 2026 Apr;16(4):e70651

Development of a prognostic model for hepatocellular carcinoma based on microvascular invasion characteristic genes by spatial transcriptomics sequencing.

Mu X, Pan L, Wang X, Liu C, Li Y, Cai Y, He Z
Frontiers in immunology 2025;16:1529569

N-terminal α-amino SUMOylation promotes phosphorylation-independent cofilin-1 translocation to the mitochondrial matrix and induces apoptosis.

Deng Q, Gu X, Feng J, Xiang J, Li X, Weng W, Zamponi GW, Huang O, Pan SJ, Li Y
Nature communications 2025 Nov 27;16(1):11687

Mechanism of adenosine monophosphate-activated protein kinase promoting lower extremity varicose veins development via cytoskeletal dynamics.

Zhang Y, Ju Z, Dai K, Wan L, Zhou G, Ji Y, Qiu J
BMC cardiovascular disorders 2025 May 31;25(1):423

Fumonisin B1 Exerts Immunosuppressive Effects Through Cytoskeleton Remodeling and Function Attenuation of Mature Dendritic Cells.

Yu Y, Zhao X, Cheng Y, Shang G, Tang K, Wang Y, Peng X, Ou S, Hu Z
International journal of molecular sciences 2025 Mar 21;26(7)

Trailer Hitch coordinates P-body organization and facilitates transcript-specific mRNA regulation through nuclear actin-mediated feedback loop.

Milano SN, Bayer LV, Ko JJ, Posner GS, Casella CE, Bratu DP
bioRxiv : the preprint server for biology 2025 Jun 20;

12/15-Lipoxygenase-Derived Electrophilic Lipid Modifications in Phagocytic Macrophages.

Deng K, Isobe Y, Tsumagari K, Kato T, Arai H, Imami K, Arita M
ACS chemical biology 2025 Feb 21;20(2):357-368

Tenvermectin B, a novel macrocyclic lactone antibiotic, suppresses glioblastoma progression by targeting RhoJ.

Nie C, Liang H, Zhou J, Liu H, Shang R, Yang H, Jiang W, Qi H, Wang J, Gao A
Cellular signalling 2025 Dec;136:112117

Involvement of Integrin β3 O-Glycosylation in Human Cytomegalovirus Internalization Into Fibroblasts.

Zheng L, Pan T, Wang H, Tian J, He Z
Journal of medical virology 2025 Aug;97(8):e70536

Protein arginine methyltransferase 1 stimulates basal cell proliferation and migration to maintain corneal epithelial homeostasis.

Yang J, Hu M, Yang M, Ni H, Zhou J, Li D, Ran J, Liu M
Cell death discovery 2025 Aug 15;11(1):385

High expression of LIMD2 predicts a poor prognosis and promotes migration of colon cancer cells.

Wang J, Cai F, Liu G, Zhang J, Li D, Cai Y, Wang Y, Xu W, Wang X, Li X
Discover oncology 2025 Aug 14;16(1):1550

Inhibition of anlotinib-induced autophagy attenuates invasion and migration by regulating epithelial-mesenchymal transition and cytoskeletal rearrangement through ATG5 in human osteosarcoma cells.

Zheng B, Sun X, Zhang L, Qu G, Ren C, Yan P, Zhou C, Yue B
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2024;57:e13152

The interaction of PRDX1 with Cofilin promotes oral squamous cell carcinoma metastasis.

Shen Y, You Z, Li L, Tang X, Shan X
International journal of cancer 2024 Oct 1;155(7):1290-1302

Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling.

Du N, Li D, Zhao W, Liu Y
Molecular biotechnology 2024 Nov;66(11):3369-3381

Neuronal CFL1 upregulation in head and neck squamous cell carcinoma enhances tumor-nerve crosstalk and promotes tumor growth.

Liu R, Wang C, Sun Z, Shi X, Zhang Z, Luo J
Molecular carcinogenesis 2024 May;63(5):874-884

PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma.

Li Q, Li J, Wang J, Wang J, Lu T, Jia Y, Sun H, Ma X
Molecular carcinogenesis 2024 Feb;63(2):253-265

METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1.

Yang R, Yang C, Su D, Song Y, Min J, Qian Z, Shen X, Li J, Su H
Cancer gene therapy 2024 Apr;31(4):562-573

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.

Weng W, Gu X, Yang Y, Zhang Q, Deng Q, Zhou J, Cheng J, Zhu MX, Feng J, Huang O, Li Y
Nature communications 2023 Sep 14;14(1):5688

ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells.

Zhang B, Ding J, Ma Z
Viruses 2023 Jun 27;15(7)

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling.

Zhu H, Wen Z, Zhang A, Liu D, Wang H, Cheng Y, Yang X, Xiao Y, Li J, Sun D, Wu B, Gao J
Communications biology 2023 Feb 23;6(1):214

HIF1A-repressed PUS10 regulates NUDC/Cofilin1 dependent renal cell carcinoma migration by promoting the maturation of miR-194-5p.

Luo W, Xu Z, Wang H, Lu Z, Ding L, Wang R, Xie H, Zheng Q, Lin Y, Zhou Z, Li Y, Chen X, Li G, Xia L
Cell & bioscience 2023 Aug 18;13(1):153

An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms.

Zhang N, Zhang H, Liu Z, Dai Z, Wu W, Zhou R, Li S, Wang Z, Liang X, Wen J, Zhang X, Zhang B, Ouyang S, Zhang J, Luo P, Li X, Cheng Q
Cell proliferation 2023 Apr;56(4):e13409

TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis.

Meng Z, Li Z, Xie M, Yu H, Jiang L, Yao X
Nature communications 2022 Sep 29;13(1):5728

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Zhang B, Fan M, Fan J, Luo Y, Wang J, Wang Y, Liu B, Sun Y, Zhao Q, Hiscox JA, Nan Y, Zhou EM
Microbiology spectrum 2022 Feb 23;10(1):e0226521

NLRP2 inhibits cell proliferation and migration by regulating EMT in lung adenocarcinoma cells.

Li T, Li X, Mao R, Pan L, Que Y, Zhu C, Jin L, Li S
Cell biology international 2022 Apr;46(4):588-598

NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.

Liu M, Xu Z, Zhang C, Yang C, Feng J, Lu Y, Zhang W, Chen W, Xu X, Sun X, Yang M, Liu W, Zhou T, Yang Y
Frontiers in cell and developmental biology 2021;9:671233

Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.

Damato M, Cardon T, Wisztorski M, Fournier I, Pieragostino D, Cicalini I, Salzet M, Vergara D, Maffia M
International journal of molecular sciences 2021 May 15;22(10)

JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.

Liu K, Cui K, Feng H, Li R, Lin H, Chen Y, Zhang Y, Chen Z, Yuan H, Li M, Wang T, Lan R, Liu J, Rao K, Wen B
Andrology 2020 Mar;8(2):497-508

No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Liu RX, Ma J, Wang B, Tian T, Guo N, Liu SJ
Neural regeneration research 2020 Jul;15(7):1290-1299

Carbonic Anhydrase XII Expression Is Modulated during Epithelial Mesenchymal Transition and Regulated through Protein Kinase C Signaling.

Vergara D, Ravaioli S, Fonzi E, Adamo L, Damato M, Bravaccini S, Pirini F, Gaballo A, Barbano R, Pasculli B, Franck J, Fournier I, Salzet M, Maffia M
International journal of molecular sciences 2020 Jan 22;21(3)

Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.

Zhang X, Fang J, Chen S, Wang W, Meng S, Liu B
Cancer medicine 2019 Sep;8(12):5716-5734

Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.

Cui K, Li R, Chen R, Li M, Wang T, Yang J, Chen Z, Wang S, Liu J, Rao K
Andrologia 2018 Feb;50(1)

Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1.

Cui K, Luan Y, Wang T, Zhuan L, Rao K, Wang SG, Ye ZQ, Liu JH, Wang DW
Asian journal of andrology 2017 Jan-Feb;19(1):67-72

Rabies virus inactivates cofilin to facilitate viral budding and release.

Zan J, An ST, Mo KK, Zhou JW, Liu J, Wang HL, Yan Y, Liao M, Zhou JY
Biochemical and biophysical research communications 2016 Sep 2;477(4):1045-1050

The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy.

Wu Q, Jiang Y, Cui S, Wang Y, Wu X
Oncology reports 2016 May;35(5):2743-54

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Zhang C, Zhang W, Lu Y, Yan X, Yan X, Zhu X, Liu W, Yang Y, Zhou T
Cell research 2016 Feb;26(2):239-53

A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.

Cao HH, Zhang SY, Shen JH, Wu ZY, Wu JY, Wang SH, Li EM, Xu LY
Oncotarget 2015 Mar 10;6(7):5435-48

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma.

Yang X, Zou P, Yao J, Yun D, Bao H, Du R, Long J, Chen X
Journal of proteome research 2010 Mar 5;9(3):1402-15

The expression of CFL1 and N-WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features.

Wang WS, Zhong HJ, Xiao DW, Huang X, Liao LD, Xie ZF, Xu XE, Shen ZY, Xu LY, Li EM
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus 2010 Aug;23(6):512-21

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP(CFL1 antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human cervical cancer using 10960-1-AP(CFL1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Cofilin antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Cofilin. This antibody recognizes human,mouse,rat antigen. The Cofilin antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10960-1-AP.pdf

: Show more