Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PB9033 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cofilin/CFL1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for COFILIN/CFL1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC/IF, ELISA. Reactive species: Human. COFILIN/CFL1 information: Molecular Weight: 18502 MW; Subcellular Localization: Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide; Tissue Specificity: Widely distributed in various tissues.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references The marine natural product, dicitrinone B, induces apoptosis through autophagy blockade in breast cancer.
The Inflammatory Transcription Factor C/EBPβ Plays a Critical Role in Cardiac Fibroblast Differentiation and a Rat Model of Cardiac Fibrosis Induced by Autoimmune Myocarditis.
Liu Q, Yang Y, Cheng M, Cheng F, Chen S, Zheng Q, Sun Y, Chen L
International journal of molecular medicine 2022 Oct;50(4)
International journal of molecular medicine 2022 Oct;50(4)
The Inflammatory Transcription Factor C/EBPβ Plays a Critical Role in Cardiac Fibroblast Differentiation and a Rat Model of Cardiac Fibrosis Induced by Autoimmune Myocarditis.
Li X, Sun M, Men S, Shi Y, Ma L, An Y, Gao Y, Jin H, Liu W, Du Z
International heart journal 2018 Nov 28;59(6):1389-1397
International heart journal 2018 Nov 28;59(6):1389-1397
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Cofilin using anti-Cofilin antibody (PB9033). Cofilin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image