PAB7900

antibody from Abnova Corporation

Targeting: CFL1 CFL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7900

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7900, RRID:AB_1707281

Product name

CFL1 (phospho S3) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of CFL1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The ADF/cofilin family: actin-remodeling proteins.

Maciver SK, Hussey PJ
Genome biology 2002;3(5):reviews3007

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Proteins of the ADF/cofilin family: essential regulators of actin dynamics.

Bamburg JR
Annual review of cell and developmental biology 1999;15:185-230

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of Jurkat cells expressing CFL1. Blots were treated with (lanes 2, 3) or without (lane 1) lambda phosphatase for 1 hr at 37 °C. In lane 3, phosphatase treatments were performed in the presence of phospho-CFL1 (Ser-3) peptide. Blots were probed with CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900) (lane 1). Phosphatase treatment removes Ser3 phosphorylation of CFL1, but this phosphatase activity is reduced in the presence of peptide.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemical labeling in chick dorsal root ganglion neurons using CFL1 (phospho S3) polyclonal antibody (Cat # PAB7900). (Images provided by Dr. Diane Snow, Department of Anatomy & Neurobiology, University of Kentucky).

Validation comment

Immunofluorescence