Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 66057-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#66057-1-Ig, RRID:AB_11043339
- Product name
- Cofilin antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated Cofilin antibody (Cat. #66057-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, pig, rabbit and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1G6A2
- Vial size
- 20ul, 150ul
Submitted references HSPB6 Deficiency Promotes the Development of Aortic Dissection and Rupture.
N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.
EMT induces characteristic changes of Rho GTPases and downstream effectors with a mitosis-specific twist.
RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.
Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.
FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.
Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein.
Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells.
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Gao S, Zhang K, Zhou C, Song J, Gu Y, Cao F, Wang J, Xie E, Yu C, Qiu J
Laboratory investigation; a journal of technical methods and pathology 2024 Mar;104(3):100326
Laboratory investigation; a journal of technical methods and pathology 2024 Mar;104(3):100326
N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.
Weng W, Gu X, Yang Y, Zhang Q, Deng Q, Zhou J, Cheng J, Zhu MX, Feng J, Huang O, Li Y
Nature communications 2023 Sep 14;14(1):5688
Nature communications 2023 Sep 14;14(1):5688
EMT induces characteristic changes of Rho GTPases and downstream effectors with a mitosis-specific twist.
Hosseini K, Frenzel A, Fischer-Friedrich E
Physical biology 2023 Sep 12;20(6)
Physical biology 2023 Sep 12;20(6)
RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.
Shimizu M, Shiraishi N, Tada S, Sasaki T, Beck G, Nagano S, Kinoshita M, Sumi H, Sugimoto T, Ishida Y, Koda T, Ishikura T, Sugiyama Y, Kihara K, Kanakura M, Nakajima T, Takeda S, Takahashi MP, Yamashita T, Okuno T, Mochizuki H
Science advances 2023 Nov 24;9(47):eadg3193
Science advances 2023 Nov 24;9(47):eadg3193
Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.
Gatford NJF, Deans PJM, Duarte RRR, Chennell G, Sellers KJ, Raval P, Srivastava DP
Human molecular genetics 2022 Mar 3;31(5):674-691
Human molecular genetics 2022 Mar 3;31(5):674-691
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Kallergi E, Daskalaki AD, Kolaxi A, Camus C, Ioannou E, Mercaldo V, Haberkant P, Stein F, Sidiropoulou K, Dalezios Y, Savitski MM, Bagni C, Choquet D, Hosy E, Nikoletopoulou V
Nature communications 2022 Feb 3;13(1):680
Nature communications 2022 Feb 3;13(1):680
Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.
Yamahashi Y, Lin YH, Mouri A, Iwanaga S, Kawashima K, Tokumoto Y, Watanabe Y, Faruk MO, Zhang X, Tsuboi D, Nakano T, Saito N, Nagai T, Yamada K, Kaibuchi K
Molecular psychiatry 2022 Aug;27(8):3479-3492
Molecular psychiatry 2022 Aug;27(8):3479-3492
FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.
Jiao X, Wang B, Yang L, Zhao Q, Zhang M, Liu X, Zhou C, Wang R, Chen H, Wang J, Ren Y, Liu P
Cell death discovery 2022 Apr 4;8(1):155
Cell death discovery 2022 Apr 4;8(1):155
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Li C, Chen L, Wang Y, Wang T, Di D, Zhang H, Zhao H, Shen X, Guo J
International journal of nanomedicine 2021;16:1663-1680
International journal of nanomedicine 2021;16:1663-1680
Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Marco S, Neilson M, Moore M, Perez-Garcia A, Hall H, Mitchell L, Lilla S, Blanco GR, Hedley A, Zanivan S, Norman JC
Nature communications 2021 Nov 24;12(1):6829
Nature communications 2021 Nov 24;12(1):6829
Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.
Creighton BA, Afriyie S, Ajit D, Casingal CR, Voos KM, Reger J, Burch AM, Dyne E, Bay J, Huang JK, Anton ES, Fu MM, Lorenzo DN
eLife 2021 Nov 23;10
eLife 2021 Nov 23;10
Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein.
Li Y, Zhang C, Lu N, Deng X, Zang G, Zhang S, Tang H, Zhang G
Frontiers in microbiology 2019;10:95
Frontiers in microbiology 2019;10:95
Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells.
Bagnato P, Castagnino A, Cortese K, Bono M, Grasso S, Bellese G, Daniele T, Lundmark R, Defilippi P, Castagnola P, Tacchetti C
Oncotarget 2017 Sep 1;8(36):60109-60122
Oncotarget 2017 Sep 1;8(36):60109-60122
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
Cui K, Ruan Y, Wang T, Rao K, Chen Z, Wang S, Liu J
The journal of sexual medicine 2017 Mar;14(3):323-335
The journal of sexual medicine 2017 Mar;14(3):323-335
MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.
Sheng N, Tan G, You W, Chen H, Gong J, Chen D, Zhang H, Wang Z
Cancer medicine 2017 Jun;6(6):1331-1340
Cancer medicine 2017 Jun;6(6):1331-1340
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Sivadasan R, Hornburg D, Drepper C, Frank N, Jablonka S, Hansel A, Lojewski X, Sterneckert J, Hermann A, Shaw PJ, Ince PG, Mann M, Meissner F, Sendtner M
Nature neuroscience 2016 Dec;19(12):1610-1618
Nature neuroscience 2016 Dec;19(12):1610-1618
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66057-1-Ig(CFL1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CFL1 antibody 66057-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
