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- Antibody Data
- Antigen structure
- References [30]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 66057-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#66057-1-Ig, RRID:AB_11043339
- Product name
- Cofilin antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated Cofilin antibody (Cat. #66057-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, pig, rabbit and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1G6A2
- Vial size
- 20ul, 150ul
Submitted references Deubiquitinase USP8 regulates the spindle assembly checkpoint in oocytes.
Pathogenic human autoantibodies against NMDA receptor alter dendritic spine size.
N-terminal α-amino SUMOylation promotes phosphorylation-independent cofilin-1 translocation to the mitochondrial matrix and induces apoptosis.
Smooth muscle LRRC8A knockout preserves vascular function in AngII hypertension.
Integrin β3 N125 glycosylation is essential for human cytomegalovirus entry into fibroblasts.
METTL5-mediated 18S rRNA m(6)A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury.
ARHGAP26 deficiency drives the oocyte aneuploidy and early embryonic development failure.
A Microtubule-Associated Protein Functions in Preventing Oocytes from Evading the Spindle Assembly Checkpoint.
Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders.
The 419th Aspartic Acid of Neural Membrane Protein Enolase 2 Is a Key Residue Involved in the Axonal Growth of Motor Neurons Mediated by Interaction between Enolase 2 Receptor and Extracellular Pgk1 Ligand.
PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior.
HSPB6 Deficiency Promotes the Development of Aortic Dissection and Rupture.
Inhibiting the SARM1-NAD(+) axis reduces oxidative stress-induced damage to retinal and nerve cells.
Human T-cell receptor triggering requires inactivation of Lim kinase-1 by Slingshot-1 phosphatase.
Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites.
N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.
EMT induces characteristic changes of Rho GTPases and downstream effectors with a mitosis-specific twist.
RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.
Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.
FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.
Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein.
Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells.
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Zhou 周长银 C, Zhang 张雪 X, Xu 许根露 G, Wang 王慧 H, Ran 冉宇婷 Y, Li 李昂 A, Sun 孙青原 QY, Ou 欧湘红 XH
Science advances 2026 Mar 13;12(11):eaeb2345
Science advances 2026 Mar 13;12(11):eaeb2345
Pathogenic human autoantibodies against NMDA receptor alter dendritic spine size.
Matsuda S, Mori T, Kasai M, Kohyama K, Shimada T, Sakuma H
Scientific reports 2026 Jan 3;16(1):3851
Scientific reports 2026 Jan 3;16(1):3851
N-terminal α-amino SUMOylation promotes phosphorylation-independent cofilin-1 translocation to the mitochondrial matrix and induces apoptosis.
Deng Q, Gu X, Feng J, Xiang J, Li X, Weng W, Zamponi GW, Huang O, Pan SJ, Li Y
Nature communications 2025 Nov 27;16(1):11687
Nature communications 2025 Nov 27;16(1):11687
Smooth muscle LRRC8A knockout preserves vascular function in AngII hypertension.
Choi H, Panja S, Nguyen HN, Stark RJ, Lamb FS
bioRxiv : the preprint server for biology 2025 May 14;
bioRxiv : the preprint server for biology 2025 May 14;
Integrin β3 N125 glycosylation is essential for human cytomegalovirus entry into fibroblasts.
Zheng L, Pan T, Wang H, He Z, Tian J
International journal of biological macromolecules 2025 Jun;313:144322
International journal of biological macromolecules 2025 Jun;313:144322
METTL5-mediated 18S rRNA m(6)A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury.
Li Z, Jiang A, Fang J, Jiang Y, He W, Yan L, Qiu S, Qin B, Zhu Q, Wang H
Experimental neurology 2025 Jan;383:115000
Experimental neurology 2025 Jan;383:115000
ARHGAP26 deficiency drives the oocyte aneuploidy and early embryonic development failure.
Li S, Zhang Y, Yuan R, Zhu S, Bai J, Miao Y, Ou X, Wang Q, Xiong B
Cell death and differentiation 2025 Feb;32(2):291-305
Cell death and differentiation 2025 Feb;32(2):291-305
A Microtubule-Associated Protein Functions in Preventing Oocytes from Evading the Spindle Assembly Checkpoint.
Zhou C, Zhang X, Xu G, Ran Y, Wang H, Xie X, Li A, Li F, Li X, Ding J, Zhang M, Sun QY, Ou XH
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Feb;12(7):e2413097
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Feb;12(7):e2413097
Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders.
Li X, Cheng R, Naeem M, Nie X, Wang J, Zhao L, Liu X, Shi Z, Zhang J
CNS neuroscience & therapeutics 2025 Apr;31(4):e70369
CNS neuroscience & therapeutics 2025 Apr;31(4):e70369
The 419th Aspartic Acid of Neural Membrane Protein Enolase 2 Is a Key Residue Involved in the Axonal Growth of Motor Neurons Mediated by Interaction between Enolase 2 Receptor and Extracellular Pgk1 Ligand.
Lee BC, Tsai JC, Huang YH, Wang CC, Lee HC, Tsai HJ
International journal of molecular sciences 2024 Oct 6;25(19)
International journal of molecular sciences 2024 Oct 6;25(19)
PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior.
Yu L, Zeng F, Fan M, Zhang K, Duan J, Tan Y, Liao P, Wen J, Wang C, Wang M, Yuan J, Pang X, Huang Y, Zhang Y, Li JD, Zhang Z, Hu Z
Zoological research 2024 May 18;45(3):535-550
Zoological research 2024 May 18;45(3):535-550
HSPB6 Deficiency Promotes the Development of Aortic Dissection and Rupture.
Gao S, Zhang K, Zhou C, Song J, Gu Y, Cao F, Wang J, Xie E, Yu C, Qiu J
Laboratory investigation; a journal of technical methods and pathology 2024 Mar;104(3):100326
Laboratory investigation; a journal of technical methods and pathology 2024 Mar;104(3):100326
Inhibiting the SARM1-NAD(+) axis reduces oxidative stress-induced damage to retinal and nerve cells.
Zhang Y, Yao Y, Yang J, Zhou B, Zhu Y
International immunopharmacology 2024 Jun 15;134:112193
International immunopharmacology 2024 Jun 15;134:112193
Human T-cell receptor triggering requires inactivation of Lim kinase-1 by Slingshot-1 phosphatase.
Gómez-Morón Á, Alegre-Gómez S, Ramirez-Muñoz R, Hernaiz-Esteban A, Carrasco-Padilla C, Scagnetti C, Aguilar-Sopeña Ó, García-Gil M, Borroto A, Torres-Ruiz R, Rodriguez-Perales S, Sánchez-Madrid F, Martín-Cófreces NB, Roda-Navarro P
Communications biology 2024 Jul 30;7(1):918
Communications biology 2024 Jul 30;7(1):918
Thermostability-assisted limited proteolysis-coupled mass spectrometry for capturing drug target proteins and sites.
Yang L, Guo CW, Luo QM, Guo ZF, Chen L, Ishihama Y, Li P, Yang H, Gao W
Analytica chimica acta 2024 Jul 11;1312:342755
Analytica chimica acta 2024 Jul 11;1312:342755
N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization.
Weng W, Gu X, Yang Y, Zhang Q, Deng Q, Zhou J, Cheng J, Zhu MX, Feng J, Huang O, Li Y
Nature communications 2023 Sep 14;14(1):5688
Nature communications 2023 Sep 14;14(1):5688
EMT induces characteristic changes of Rho GTPases and downstream effectors with a mitosis-specific twist.
Hosseini K, Frenzel A, Fischer-Friedrich E
Physical biology 2023 Sep 12;20(6)
Physical biology 2023 Sep 12;20(6)
RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS.
Shimizu M, Shiraishi N, Tada S, Sasaki T, Beck G, Nagano S, Kinoshita M, Sumi H, Sugimoto T, Ishida Y, Koda T, Ishikura T, Sugiyama Y, Kihara K, Kanakura M, Nakajima T, Takeda S, Takahashi MP, Yamashita T, Okuno T, Mochizuki H
Science advances 2023 Nov 24;9(47):eadg3193
Science advances 2023 Nov 24;9(47):eadg3193
Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.
Gatford NJF, Deans PJM, Duarte RRR, Chennell G, Sellers KJ, Raval P, Srivastava DP
Human molecular genetics 2022 Mar 3;31(5):674-691
Human molecular genetics 2022 Mar 3;31(5):674-691
Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.
Kallergi E, Daskalaki AD, Kolaxi A, Camus C, Ioannou E, Mercaldo V, Haberkant P, Stein F, Sidiropoulou K, Dalezios Y, Savitski MM, Bagni C, Choquet D, Hosy E, Nikoletopoulou V
Nature communications 2022 Feb 3;13(1):680
Nature communications 2022 Feb 3;13(1):680
Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.
Yamahashi Y, Lin YH, Mouri A, Iwanaga S, Kawashima K, Tokumoto Y, Watanabe Y, Faruk MO, Zhang X, Tsuboi D, Nakano T, Saito N, Nagai T, Yamada K, Kaibuchi K
Molecular psychiatry 2022 Aug;27(8):3479-3492
Molecular psychiatry 2022 Aug;27(8):3479-3492
FMNL2 suppresses cell migration and invasion of breast cancer: a reduction of cytoplasmic p27 via RhoA/LIMK/Cofilin pathway.
Jiao X, Wang B, Yang L, Zhao Q, Zhang M, Liu X, Zhou C, Wang R, Chen H, Wang J, Ren Y, Liu P
Cell death discovery 2022 Apr 4;8(1):155
Cell death discovery 2022 Apr 4;8(1):155
Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.
Li C, Chen L, Wang Y, Wang T, Di D, Zhang H, Zhao H, Shen X, Guo J
International journal of nanomedicine 2021;16:1663-1680
International journal of nanomedicine 2021;16:1663-1680
Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.
Marco S, Neilson M, Moore M, Perez-Garcia A, Hall H, Mitchell L, Lilla S, Blanco GR, Hedley A, Zanivan S, Norman JC
Nature communications 2021 Nov 24;12(1):6829
Nature communications 2021 Nov 24;12(1):6829
Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A.
Creighton BA, Afriyie S, Ajit D, Casingal CR, Voos KM, Reger J, Burch AM, Dyne E, Bay J, Huang JK, Anton ES, Fu MM, Lorenzo DN
eLife 2021 Nov 23;10
eLife 2021 Nov 23;10
Involvement of Actin-Regulating Factor Cofilin in the Inclusion Body Formation and RNA Synthesis of Human Parainfluenza Virus Type 3 via Interaction With the Nucleoprotein.
Li Y, Zhang C, Lu N, Deng X, Zang G, Zhang S, Tang H, Zhang G
Frontiers in microbiology 2019;10:95
Frontiers in microbiology 2019;10:95
Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells.
Bagnato P, Castagnino A, Cortese K, Bono M, Grasso S, Bellese G, Daniele T, Lundmark R, Defilippi P, Castagnola P, Tacchetti C
Oncotarget 2017 Sep 1;8(36):60109-60122
Oncotarget 2017 Sep 1;8(36):60109-60122
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
Cui K, Ruan Y, Wang T, Rao K, Chen Z, Wang S, Liu J
The journal of sexual medicine 2017 Mar;14(3):323-335
The journal of sexual medicine 2017 Mar;14(3):323-335
MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway.
Sheng N, Tan G, You W, Chen H, Gong J, Chen D, Zhang H, Wang Z
Cancer medicine 2017 Jun;6(6):1331-1340
Cancer medicine 2017 Jun;6(6):1331-1340
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
Sivadasan R, Hornburg D, Drepper C, Frank N, Jablonka S, Hansel A, Lojewski X, Sterneckert J, Hermann A, Shaw PJ, Ince PG, Mann M, Meissner F, Sendtner M
Nature neuroscience 2016 Dec;19(12):1610-1618
Nature neuroscience 2016 Dec;19(12):1610-1618
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 66057-1-Ig(CFL1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CFL1 antibody 66057-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
