Ab097098

antibody from Aladdin Scientific

Targeting: CFL1 CFL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab097098

Provider

Aladdin Scientific

Product name

Cofilin 1 Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human Cofilin 1 Antibody; Monoclonal antibody (C12), could be used for WB, IHC and so on.ApplicationWB: 0.2-2µg/mLIHC: 5-20µg/mLIF/ICC: 5-30µg/mLPurityProtein A + Protein G affinity chromatographyFunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Ser3~Leu161


Antibody clone number

C12

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at 4¡C short term. Aliquot and store at -20¡C long term_24 months_. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cofilin 1 Mouse mAb (Ab097098) - Western Blot All lanes: Cofilin 1 Mouse mAb (Ab097098) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 1.0 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cofilin 1 Mouse mAb (Ab097098) - IF/ICC AF488 staining on IF Sample: Human HeLa cell Primary Ab: 30 µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2 µg/mL AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Small intestine Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody