MA5-13016

antibody from Invitrogen Antibodies

Targeting: ERBB4 ALS19, HER4

Western blot

Antibody data

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Validation data

Product number: MA5-13016 - Provider product page
Provider: Invitrogen Antibodies
Product name: ErbB4 Monoclonal Antibody (H4.72.8 (Ab72))
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-13016 targets HER-4 in WB, FACS applications and shows reactivity with Human and Mouse samples. The MA5-13016 immunogen is extracellular domain of recombinant human c-erbB-4/HER-4 oncoprotein.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: H4.72.8 (Ab72)
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

ERBB4 protein structure - MA5-13016 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of HER-4 in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of HER-4 in Neuro-2a cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of HER-4 in T47D cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a HER-4 monoclonal antibody (Product # MA5-13016) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.