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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F48403 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-ERBB4 Antibody (pY1162)
- Antibody type
- Polyclonal
- Antigen
- This phospho-ERBB4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1162 of human ERBB4.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-ERBB4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of phospho-ERBB4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of phospho-ERBB4 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with the phospho-ERBB4 antibody.
