PA5-121121

antibody from Invitrogen Antibodies

Targeting: GLUD1 GDH, GLUD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121121

Provider

Invitrogen Antibodies

Product name

GluD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HeLa, HepG2, NIH/3T3, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain, Rat kidney. The target is usually found in the following locations: Mitochondrion matrix. Immunogen sequence: SEAVADREDD PNFFKMVEGF FDRGASIVED KLVEDLRTRE SEEQKRNRVR GILRIIKPCN HVLSLSFPIR RDDGSWEVIE GYRAQHSQHR TPCKGGIRYS TDVSVDEVKA LASLMTYKCA VVDVPFGGAK AGVKINPKNY TDNELEKITR RFTMELAKKG FIGPGIDVPA PDMSTGEREM SWIADTYAS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.02 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GluD1 in extracts of various cell lines. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GluD1 in C6 cells. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GluD1 in C6 cells using a GluD1 Polyclonal antibody (Product # PA5-121121). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GluD1 in C6 cells using a GluD1 Polyclonal antibody (Product # PA5-121121). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GluD1 in NIH-3T3 cells. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GluD1 in C6 cells. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GluD1 in 300 μg extracts of HepG2 cells. Samples were precipitated with 3 μg GluD1 Polyclonal antibody (Product # PA5-121121). Western blot was performed from the immunoprecipitate using GluD1 Polyclonal antibody (Product # PA5-121121) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded human colon carcinoma. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded human liver. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded mouse kidney. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded rat kidney. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded human colon carcinoma. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded human liver. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded mouse kidney. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GluD1 in paraffin-embedded rat kidney. Samples were incubated with GluD1 Polyclonal antibody (Product # PA5-121121) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.