PA5-110928

antibody from Invitrogen Antibodies

Targeting: GPR182 ADMR, AM-R, G10D, hrhAMR

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110928

Provider

Invitrogen Antibodies

Product name

GPR182 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LSPHFRGRLL NAVVHYLPKD QTKAGTCASS SSCSTQHSII ITKGDSQPAA AAPHPEPSLS FQAHHLLPNT SPISPTQPLT PS

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

GPR182 limits antitumor immunity via chemokine scavenging in mouse melanoma models.

Torphy RJ, Sun Y, Lin R, Caffrey-Carr A, Fujiwara Y, Ho F, Miller EN, McCarter MD, Lyons TR, Schulick RD, Kedl RM, Zhu Y
Nature communications 2022 Jan 10;13(1):97

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 GPR182 is expressed by lymphatic endothelial cells in human melanoma. a , b Two published single-cell RNA sequencing datasets of human melanoma were queried for GPR182 expression based on cell type ( a ). b Analysis of ECs in human melanoma further revealed that GPR182 is primarily expressed in LECs. c Human melanoma tissues were stained for GPR182 (green) together with EC markers, including CD31, podoplanin (PDPN), or Prox-1. d A lymphatic score was generated using 288 metastatic melanoma samples from the TCGA database. The lymphatic score was calculated based on relative expression levels of PDPN , LYVE1 , and VEGFC in each sample. Lymphatic score was plotted against GPR182 mRNA expression level (RSEM, log2 normalized). Pearson's correlation coefficient, r , and p -values shown from two-sided test. e , f Human melanoma tissues with adjacent normal skin were stained for PDPN (red), CD31(blue), and GPR182 (green) (E); S-100 staining (brown) was used to identify melanocytes and tumor cells. f Quantification of GPR182 median fluorescent intensity (MFI) in PDPN+ lymphatic vessels from paired tumor and adjacent normal tissue. n = 15, P -value from two-sided paired t -test. Error bars represent SEM.