PA5-78246

antibody from Invitrogen Antibodies

Targeting: TRRAP PAF400, TR-AP, Tra1

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78246

Provider

Invitrogen Antibodies

Product name

TRRAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TRRAP in A) Jurkat cells and B) Raji cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with TRRAP polyclonal antibody (Product # PA5-78246) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TRRAP in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with TRRAP polyclonal antibody (Product # PA5-78246) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRRAP Polyclonal Antibody detects TRRAP protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. TRRAP Polyclonal Antibody (Product # PA5-78246) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRRAP Polyclonal Antibody detects TRRAP protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.5 % SDS-PAGE. TRRAP Polyclonal Antibody (Product # PA5-78246) dilution: 1:1,000.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of TRRAP in HeLa chromatin extract using 5 µg of sample. Immunoprecipitation was performed using TRRAP polyclonal antibody (Product # PA5-78246). Analysis of the precipitated DNA was done by PCR using primer targeting nucleolin promoter.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or TRRAP Polyclonal Antibody (Product # PA5-78246). The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.