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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- GTX116478 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116478, RRID:AB_10615505
- Product name
- PRMT3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX116478 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX116478 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody (GTX116478) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments.IP samples: HeLa whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of PRMT3 protein by 4 £gg PRMT3 antibody (GTX116478)7.5 % SDS-PAGEThe immunoprecipitated PRMT3 protein was detected by PRMT3 antibody (GTX116478) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
