Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-34752B - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: Cytokeratin 10 Antibody (SPM261) [Biotin] [NBP2-34752B] - An intracellular stain was performed on A431 cells with Cytokeratin 10 [SPM261] Antibody NBP2-34752B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).