Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-47825-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 10 Antibody (KRT10/844) - Azide and BSA Free [NBP2-47825] - Western Blot Analysis of human Thymus tissue lysate using Cytokeratin 10 Antibody (KRT10/844).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (KRT10/844) - Azide and BSA Free [NBP2-47825] - Formalin-fixed, paraffin-embedded human skin stained with cytokeratin 10 Monoclonal Antibody (KRT10/844).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 10 Antibody (KRT10/844) - Azide and BSA Free [NBP2-47825] - An intracellular stain was performed on HT-29 cells with Cytokeratin 10 (KRT10/844) NBP2-47825F (blue) and a matched isotype control NBP1-97005F (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC. Image using the FITC form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Cytokeratin 10 Antibody (KRT10/844) - Azide and BSA Free [NBP2-47825] - An intracellular stain was performed on HeLa cells with Cytokeratin 10 Antibody (KRT10/844) NBP2-47825APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image using the Allophycocyanin form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Cytokeratin 10 Antibody (KRT10/844) - Azide and BSA Free [NBP2-47825] - An intracellular stain was performed on HeLa cells with Cytokeratin 10 Antibody (KRT10/844) NBP2-47825F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.