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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- RQ8355 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LHX6 Antibody / LIM homeobox protein 6
- Antibody type
- Polyclonal
- Description
- This highly specific LHX6 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the LHX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) 293T, 3) HepG2 and 4) Caco-2 cell lysate with LHX6 antibody. Predicted molecular weight: 38-43 kDa (multiple isoforms) but can be observed at higher molecular weights when complexed with PITX2.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with LHX6 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thyroid cancer tissue with LHX6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
