Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-52657-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD52 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-52657] - Western Blot using anti-CD52 antibody YTH 34.5-G2b (Campath-1G). Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.