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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB300-236 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-236, RRID:AB_2199153
- Product name
- Rabbit Polyclonal Synaptotagmin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.
- Reactivity
- Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding.
Different domains of synaptotagmin control the choice between kiss-and-run and full fusion.
Synaptotagmin I functions as a calcium regulator of release probability.
Regulation of synaptotagmin I phosphorylation by multiple protein kinases.
Regulation of synaptotagmin I phosphorylation by multiple protein kinases.
Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH
Molecular cell 2004 Sep 10;15(5):741-51
Molecular cell 2004 Sep 10;15(5):741-51
Different domains of synaptotagmin control the choice between kiss-and-run and full fusion.
Wang CT, Lu JC, Bai J, Chang PY, Martin TF, Chapman ER, Jackson MB
Nature 2003 Aug 21;424(6951):943-7
Nature 2003 Aug 21;424(6951):943-7
Synaptotagmin I functions as a calcium regulator of release probability.
Fernández-Chacón R, Königstorfer A, Gerber SH, García J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Südhof TC
Nature 2001 Mar 1;410(6824):41-9
Nature 2001 Mar 1;410(6824):41-9
Regulation of synaptotagmin I phosphorylation by multiple protein kinases.
Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ
Journal of neurochemistry 1999 Sep;73(3):921-32
Journal of neurochemistry 1999 Sep;73(3):921-32
Regulation of synaptotagmin I phosphorylation by multiple protein kinases.
Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ
Journal of neurochemistry 1999 Sep;73(3):921-32
Journal of neurochemistry 1999 Sep;73(3):921-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.
