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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX114124 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114124, RRID:AB_11163039
- Product name
- Synaptotagmin 1 antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX114124 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (10 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Synaptotagmin 1 antibody [N1C2] detects Synaptotagmin 1 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synaptotagmin 1 protein stained by Synaptotagmin 1 antibody [N1C2] (GTX114124) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
