PA5-29922

antibody from Invitrogen Antibodies

Targeting: SYT1 P65, SVP65, SYT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29922

Provider

Invitrogen Antibodies

Product name

Synaptotagmin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: mouse brain, rat brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of synaptotagmin 1 using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a synaptotagmin 1 polyclonal antibody (Product # PA5-29922) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Synaptotagmin 1 was performed by separating 10 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Synaptotagmin 1 Polyclonal Antibody (Product # PA5-29922) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Synaptotagmin 1 Polyclonal Antibody (Product # PA5-29922). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 Polyclonal Antibody (Product # PA5-29922) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Synaptotagmin 1 was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptotagmin 1 Polyclonal Antibody (Product # PA5 29922) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Synaptotagmin 1 Polyclonal Antibody detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Synaptotagmin 1 stained by Synaptotagmin 1 Polyclonal Antibody (Product # PA5-29922) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.