AP31821PU-N

antibody from Acris Antibodies GmbH

Targeting: SYT1 P65, SVP65, SYT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP31821PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP31821PU-N, RRID:AB_11148408

Product name

anti Synaptotagmin-1

Antibody type

Polyclonal

Antigen

Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products.Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.Antibodies were affinity-purified and mixed in equal concentrations.

Reactivity

Human, Mouse

Host

Chicken/Avian

Vial size

0.4 mg

Concentration

0.4 mg/ml. The concentrations of each of the four antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot showing a single Synaptotagmin bond running at about 65 kDa